Characterization and Immunolocalization of Bovine Uterine Retinol-Binding Protein1
نویسندگان
چکیده
منابع مشابه
Confocal immunolocalization of bovine serum albumin, serum retinol-binding protein, and interphotoreceptor retinoid-binding protein in bovine retina.
PURPOSE Recently it has been shown that the transport as well as clearance of retinol from isolated rod photoreceptors requires an extracellular factor. Interphotoreceptor retinoid-binding protein (IRBP) is a component of the interphotoreceptor matrix (IPM) and is known to bind visual cycle retinoids. Serum albumin and serum retinol-binding protein (sRBP), proteins capable of binding retinoids,...
متن کاملCharacterization of bovine plasma retinol-binding protein and evidence for lack of binding between it and other bovine plasma proteins.
Bovine retinol-retinol-binding protein (RBP) was isolated from serum as a free, uncomplexed protein under experimental conditions in which human, rabbit, and chicken retinol-RBP are present as tight complexes with prealbumin (thyroxine-binding protein). Purified bovine retinol-RBP formed tight complexes with purified human and chicken prealbumin in physiological ionic strength buffers as judged...
متن کاملCellular Retinol- and Retinoic Acid-binding Proteins of Bovine Retina
A three-step purification has been developed for the cellular retinoland cellular retinoic acid-binding proteins from extracts of bovine retina. The two binding proteins elute together in Step 1 (gel filtration) and separate in Step 2 (DEAE-cellulose chromatography), and each is purified to apparent homogeneity in the third step (calcium phosphate gel chromatography). The retinoland retinoic ac...
متن کاملInteractions of plasma retinol-binding protein with its receptor. Specific binding of bovine and human retinol-binding protein to pigment epithelium cells from bovine eyes.
Bovine and human retinol-retinol-binding protein (RBP) were iodinated to high specific activity. At least 34% of the iodinated material was native and retained its retinol chromophore as judged by its characteristic ability to bind to plasma prealbumin. Bovine and human retinol-125I-RBP were found to bind specifically to intact isolated pigment epithelium cells from bovine eyes. The specific bi...
متن کاملMolecular properties of bovine interphotoreceptor retinol-binding protein.
Interphotoreceptor retinol-binding protein (IRBP) is a large retinol-carrying glycoprotein, located only in the interphotoreceptor (or subretinal) space of vertebrate eyes. It has recently been purified to apparent homogeneity. The present report presents its sedimentation, spectroscopic, and binding properties. The molecular weight of bovine IRBP, determined by sedimentation equilibrium, is 13...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1992
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod47.6.1099